It also showed as much as a tenfold improvement in sensitivity with labile peptides on standard stainless-steel targets. This matrix was found to be better performing than CHCA in overall sensitivity and showed better sequence coverage at low-digest levels, partly as a result of less of a bias for arginine-containing peptides. The performance of this matrix with labile peptides, such as phosphopeptides and 4-sulfophenyl isothiocynate-derivatized peptides, to facilitate de novo sequencing was also explored. The overall sequence coverage as well as sensitivity of this matrix were compared with CHCA using standard protein tryptic digests. 1 In this study, the performance of this new matrix, using the Shimadzu Biotech Axima TOF 2 (Shimadzu Biotech, Manchester, UK), was investigated. Recently, a new rationally designed matrix 4-chloro-α-cyanocinnamic acid was introduced, which is reported to have superior performance as compared with the “gold standard” α-cyano-4-hydroxycinnamic acid (CHCA). MALDI-TOF continues to be an important tool for many proteomic studies.
0 Comments
Leave a Reply. |